{"id":1821,"date":"2022-10-06T18:04:51","date_gmt":"2022-10-06T09:04:51","guid":{"rendered":"https:\/\/peptistar.com\/?page_id=1821"},"modified":"2022-10-16T11:43:03","modified_gmt":"2022-10-16T02:43:03","slug":"hilic","status":"publish","type":"page","link":"https:\/\/peptistar.com\/en\/technology\/hilic\/","title":{"rendered":"HILIC"},"content":{"rendered":"
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Peptide and Oligonucleotide API Manufacturing Technologies<\/p>\n<\/p><\/div>\n

HILIC<\/h2>\n

Development of separation analysis method using a column with complementary properties to ODS column
\n\t\t\t(HILIC, SFC, normal-phase mode)<\/h3>\n

\t\tODS column in reverse-phase HPLC mode is generally used in purification and purity evaluation of Peptides, but a separation method is needed that has high orthogonality to ODS column since there is a possibility of impurity overlapping with the main peak.
\nTo resolve this issue, PeptiStar has jointly developed a separation method that can be used for Peptide API manufacturing. It is now possible to separate impurities that were difficult to separate with conventional methods by using analysis column (DCpak P series) from Daicel Corporation in HILIC or SFC mode that enables separation that has high complementary properties to ODS column. PeptiStar utilizes either ODS column or DCPak P series in order to enable more accurate purity evaluation.\t<\/p>\n

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Chromatograph of Basic Cyclic Peptide: Comparison of reverse-phase HPLC using ODS column and HILIC mode using DCpak P series<\/h3>\n

\t\t\t\"Chromatograph\n\t\t<\/div>\n

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Advantages of HILIC Mode<\/strong><\/p>\n